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41.
《Fungal biology》2020,124(1):44-53
Alternaria blight is one of the most devastating diseases of rapeseed-mustard caused by a necrotrophic fungus Alternaria brassicae. Lack of satisfactory resistance resource in Brassica is still a main obstruction for developing resistance against Alternaria. In this study, we have selected Brassica juncea, Sinapis alba and Camelina sativa to understand and unravel the mechanism of disease resistance against Alternaria. Histopathological studies showed early onset of necrosis in B. juncea (1 dpi) and delayed in S. alba (2 dpi) and C. sativa (3 dpi) respectively. Early and enhanced production of hydrogen peroxide (H2O2) was observed in C. sativa and S. alba (6 hpi) when compared to B. juncea (12 hpi). An increase in catalase activity was observed in both C. sativa (36 % at 6 hpi) and S. alba (15 % at 12 hpi), whereas it significantly decreased in B. juncea at 6 hpi (23 %), 12 hpi (30 %) and 24 hpi (8 %). Gene expression analysis showed induction of PR-3 and PR-12 genes only in C. sativa and S. alba when compared to B. juncea suggesting their vital role for Alternaria resistance. In contrast, SA marker genes were significantly expressed in B. juncea only which provides evidence of hormonal cross talk in B. juncea during Alternaria infection thereby increasing its susceptibility. 相似文献
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Zusammenfassung Die serotoninhaltigen, nach Formaldehydbedampfung im UV-Licht gelbfluoreszierenden EC-Zellen im Magendarmepithel wurden bei normalen und bei tryptophanfrei ernährten Ratten untersucht. Bei Kontrolltieren ist die Zahl der EC-Zellen, bezogen auf die Schleimhautfläche, im Duodenum größer als im Pylorus. Im Duodenum sind die EC-Zellen etwa gleichmäßig auf Oberflächen- und Drüsenepithel verteilt, im Pylorus ganz überwiegend im Drüsenepithel lokalisiert. Die Brunnerschen Drüsen besitzen keine EC-Zellen. Tryptophanfreie Diät führt zu starker Abnahme des Serotoningehalts der EC-Zellen, nach Umsetzung auf Normalkost steigt er wieder an. — Die Ergebnisse sprechen weiterhin dafür, daß die EC-Zelle sowohl Speicher- als auch Syntheseort von Serotonin ist. Dabei kommt Serotonin in der EC-Zelle wahrscheinlich in zwei Fraktionen vor, nämlich zytoplasmatisch gelöst und granulagebunden. Die histochemischen Nachweismethoden für Serotonin, Möglichkeiten des Eingriffs in die verschiedenen Syntheseschritte des Serotonins und deren Spezifität in bezug auf die EC-Zelle als physiologischem Ort der Serotoninsynthese werden diskutiert.
Studies on the endocrine cells of the gastrointestinal tractII. Fluorescence microscopy of the EC-cells after tryptophan-free diet
Summary The serotonin-containing EC-cells of the gastro-intestinal mucosa, which show yellow fluorescence after treatment with gaseous formaldehyde, were investigated in the rat after normal and tryptophan-free nourishment. In control animals the number of EC-cells, related to epithelium area is higher in the duodenum than in the pyloric region. In the duodenum the EC-cells show about the same distribution in surface epithelium and gland epithelium. In the pyloric region EC-cells are localized predominantly in the gland epithelium. No EC-cells were found in the Brunner glands. After tryptophan-free diet the serotonin-level of the EC-cells strongly decreases; serotonin-level increases after return to normal nourishment. The results also suggest, that serotonin is synthesized as well as stored in the EC-cell and that it occurs in two fractions: bound to granules and dissolved in the cytoplasm. Discussion deals with the histochemical methods for determination of serotonin, the possibilities of influencing the pathways of serotonin metabolism and the specificity of these possibilities concerning the EC-cell as the physiological site of serotonin synthesis.
Mit Unterstützung durch die Deutsche Forschungsgemeinschaft, Antrag Fo 77, 1–4. 相似文献
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摘要 目的:探讨肺癌合并肺部感染患者病原菌分布、耐药性分析及血清炎性因子检测的临床意义。方法:选取我院于2018年6月~2020年3月期间收治的肺癌合并肺部感染患者90例为感染组,选取同期我院收治的肺癌未合并肺部感染患者100例为肺癌组,选取同期于我院进行体检的健康志愿者60例为对照组,对肺癌合并肺部感染患者的感染病原菌类型进行总结分析,分析常见病原菌药敏试验结果,统计病原菌耐药率。对比三组受试者的炎性因子水平。结果:90例肺癌合并肺部感染患者的送检痰液标本共检出100株病原菌,100株病原菌中以革兰阴性菌为主,其次为革兰阳性菌、真菌,分别占比63.00%、22.00%、15.00%。肺炎克雷伯菌、鲍氏不动杆菌、铜绿假单胞菌对亚胺培南的耐药率较低,对氨苄西林、甲氨苄啶的耐药率均较高。凝固酶阴性葡萄球菌、金黄色葡萄球菌对万古霉素的耐药率较高,对左氧氟沙星的耐药率较低。白色念珠菌、热带念珠菌对两性霉素B、氟康唑、酮康唑、伊曲康唑的耐药率均较低。感染组、肺癌组的血清白介素-6(IL-6)、降钙素原(PCT)、肿瘤坏死因子-α(TNF-α)水平均高于对照组,且感染组以上指标水平高于肺癌组(P<0.05)。结论:肺癌合并肺部感染患者体内病原菌种类繁多,对常见抗菌药物的耐药性存在差异,且患者体内存在较强的炎性反应,临床应根据药敏结果合理应用抗菌药物 。 相似文献
45.
《Critical reviews in biotechnology》2013,33(2):126-144
Sea buckthorn is a berry crop with multiple uses. The berries are highly appreciated for their unique taste but are also very rich in bioactive compounds with powerful nutritional and medicinal values. In addition, the plants grow well under adverse conditions, and are often used to fight soil erosion. Utilization of sea buckthorn has therefore increased around the world but serious problems have, nevertheless, been encountered due to drought, salinity, diseases and insect pests. This review covers important aspects of sea buckthorn research, such as heritable and environmentally induced variation in biochemical compounds, causes and effects of the devastating dried-shrink disease, susceptibility to insect pests, methods for conventional breeding, and the utilization of DNA markers for taxonomical and population genetic analyses, and for investigating the inheritance of quality and resistance traits. We also present possibilities to implement innovative biotechnological breeding methods, especially metabolite profiling and MAS/GRC-based markers, for fast and efficient development of elite genotypes with specific nutritional- and health-related bioactive compounds and strong resistance to biotic and abiotic stress. 相似文献
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K. Choi M. D. Burow G. Church G. Burow A. H. Paterson C. E. Simpson J. L. Starr 《Journal of nematology》1999,31(3):283-290
Segregation of resistance to Meloidogyne arenaria in six BC₅F₂ peanut breeding populations was examined in greenhouse tests. Chi-square analysis indicated that segregation of resistance was consistent with resistance being conditioned by a single gene in three breeding populations (TP259-3, TP262-3, and TP271-2), whereas two resistance genes may be present in the breeding populations TP259-2, TP263-2, and TP268-3. Nematode development in clonally propagated lines of resistant individuals of TP262-3 and TP263-2 was compared to that of the susceptible cultivar Florunner. Juvenile nematodes readily penetrated roots of all peanut genotypes, but rate of development was slower (P = 0.05) in the resistant genotypes than in Florunner. Host cell necrosis indicative of a hypersensitive response was not consistently observed in resistant genotypes of either population. Three RFLP loci linked to resistance at distances of 4.2 to 11.0 centiMorgans were identified. Resistant and susceptible alleles for RFLP loci R2430E and R2545E were quite distinct and are useful for identifying individuals homozygous for resistance in segregating populations. 相似文献
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N.T. Nguyen M. Liu H. Katayama T. Takemura I. Kasuga 《Letters in applied microbiology》2021,72(3):275-282
Colistin is one of the antibiotics of last resort for human health. However, the dissemination of the plasmid-mediated colistin resistance gene mcr-1 is of great concern globally. In the One Health framework, the environment is an important component for managing antimicrobial resistance. However, little information is available concerning the prevalence of mcr-1 in water environments. We aimed to reveal the prevalence of mcr-1 in different water environments in Hanoi, Vietnam. Quantitative PCR was applied to detect mcr-1 in four urban drainages receiving untreated domestic wastewater, three rivers, five lakes and two groundwater samples. Urban drainages contained higher concentrations of mcr-1, suggesting that urban residents carry the gene. The class 1 integron-integrase gene was identified as a good surrogate of antibiotic resistance genes including mcr-1. A significant correlation was found between the levels of mcr-1 and the human-specific cross-assembly phage, which is an indicator of human faecal pollution. These results indicated that the primary source of mcr-1 in urban water environments is human faeces, which is consistent with the fact that most domestic wastewater is untreated in Hanoi. The control of untreated wastewater is critical for alleviating the spread of mcr-1 in water environments in Vietnam. 相似文献